In ‘Vigyan Ashram’s ‘ microbiology lab, Tricoderma viridae is multiplied, made powder of it, packed and sold to the Farmers & also used by the agriculture department students within the Vigyan Ashram. Tricoderma is an very effective bio control agent for the crops. Hence it is in demand among Farmers, Gardeners and all people involved in agricultural Section.
In order to produce Tricoderma Powder, first we have to inoculate Tricoderma on the sterile substrate, allow it to multiply on it. For inoculation we require pure Tricoderma viridae strain.
When we plated the Tricoderma powder which was made in Ashram on PDA; it was observed that along with Greenish growth of Tricoderma viridae, some unwanted growth (unknown) was observed. So the need was raised to isolate pure form of Tricoderma viridae from the powder. To isolate Tricoderma viridae from the given powder sample by Streak plate method.
Main objectives of this project are:
To isolate Tricoderma viridae from the given powder sample by Streak plate method.
To learn and improve the plating skills.
More details of this project are here : http://vadic.vigyanashram.blog/2019/11/30/assignment-tricoderma-viridae-streaking-on-pda-plate/